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Biosensors-- are powerful tools aimed at providing selective identification of toxic chemical compounds at ultratrace levels in industrial products, chemical substances, environmental samples (e.g., air, soil, and water) or biological systems (e.g., bacteria, virus, or tissue components) for biomedical diagnosis. Combining the exquisite specificity of biological recognition probes and the excellent sensitivity of laser-based optical detection, biosensors are capable of detecting and differentiating big/chemical constituents of complex systems in order to provide unambiguous identification and accurate quantification. A new generation of biosensors discussed in this presentation uses antibody and DNA probes.